› 1dh3

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF A CREB BZIP-CRE COMPLEX REVEALS THE BASIS FOR CREB FAIMLY SELECTIVE DIMERIZATION AND DNA BINDING

Released:
DOI: 10.2210/pdb1dh3/pdb
PDB entry 1dh3 coloured by chain, front view.
PDB entry 1dh3 coloured by chain, side view.
PDB entry 1dh3 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The structure of a CREB bZIP.somatostatin CRE complex reveals the basis for selective dimerization and divalent cation-enhanced DNA binding.
Schumacher MA, Goodman RH, Brennan RG
J Biol Chem 275 35242-7 (2000)
PMID: 10952992

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113680 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Cyclic AMP-responsive element-binding protein 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 55 amino acids
Theoretical weight: 6.69 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01147 (Residues: 271-325; Coverage: 17%)
Gene names: Creb-1, Creb1
Sequence domains: bZIP transcription factor
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
DNA (5'-D(*CP*CP*TP*TP*GP*GP*CP*TP*GP*AP*CP*GP*TP*CP*AP*GP*CP*CP*AP*AP*G)-3') Chains: B, D
Molecule details ›
Chains: B, D
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 110.61Å b: 49.32Å c: 79.25Å
α: 90° β: 122.18° γ: 90°
R-values:
R R work R free
0.218 0.223 0.28
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

26 review citations

Transcriptional regulation by the phosphorylation-dependent factor CREB.
Mayr et al. (2001)
25 more

32 mentions without citation

Chemical modulation of transcription factors.
Wiedemann et al. (2018)
31 more