› 1di2

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A DSRNA-BINDING DOMAIN COMPLEXED WITH DSRNA: MOLECULAR BASIS OF DOUBLE-STRANDED RNA-PROTEIN INTERACTIONS

Released:
DOI: 10.2210/pdb1di2/pdb
PDB entry 1di2 coloured by chain, front view.
PDB entry 1di2 coloured by chain, side view.
PDB entry 1di2 coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
Molecular basis of double-stranded RNA-protein interactions: structure of a dsRNA-binding domain complexed with dsRNA.
Ryter JM, Schultz SC
EMBO J 17 7505-13 (1998)
PMID: 9857205

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-187496 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Interferon-inducible double-stranded RNA-dependent protein kinase activator A homolog B Chains: A, B
Molecule details ›
Chains: A, B
Length: 69 amino acids
Theoretical weight: 7.69 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91836 (Residues: 112-180; Coverage: 23%)
Gene names: prkra-b, rbpa
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain
RNA (5'-R(*GP*GP*CP*GP*CP*GP*CP*GP*CP*C)-3') Chains: C, D, E, G
Molecule details ›
Chains: C, D, E, G
Length: 10 nucleotides
Theoretical weight: 3.21 KDa
Source organism: Xenopus laevis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 110.4Å b: 58.5Å c: 58.9Å
α: 90° β: 105.2° γ: 90°
R-values:
R R work R free
0.231 0.228 0.258
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

81 review citations

RNA editing by adenosine deaminases that act on RNA.
Bass BL. (2002)
80 more

44 mentions without citation

RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence.
Masliah et al. (2013)
43 more