› 1djr

X-ray diffraction
1.3Å resolution

HEAT-LABILE ENTEROTOXIN B-PENTAMER COMPLEXED WITH M-CARBOXYPHENYL-ALPHA-D-GALACTOSE

Released:
DOI: 10.2210/pdb1djr/pdb
PDB entry 1djr coloured by chain, front view.
PDB entry 1djr coloured by chain, side view.
PDB entry 1djr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of m-carboxyphenyl-alpha-D-galactopyranoside complexed to heat-labile enterotoxin at 1.3 A resolution: surprising variations in ligand-binding modes.
Minke WE, Pickens J, Merritt EA, Fan E, Verlinde CL, Hol WG
Acta Crystallogr D Biol Crystallogr 56 795-804 (2000)
PMID: 10930826

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152492 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat-labile enterotoxin B chain Chains: D, E, F, G, H
Molecule details ›
Chains: D, E, F, G, H
Length: 103 amino acids
Theoretical weight: 11.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32890 (Residues: 22-124; Coverage: 100%)
Gene names: eltB, ltpB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

3 bound ligands:
Ligand GLA 5 x GLA
Ligand GOL 3 x GOL
Ligand BEZ 3 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 42.368Å b: 95.04Å c: 67.507Å
α: 90° β: 100.71° γ: 90°
R-values:
R R work R free
0.137 0.134 0.189
Expression system: Escherichia coli

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Citations

1 review citation

Stability of membrane proteins: relevance for the selection of appropriate methods for high-resolution structure determinations.
Rosenbusch JP. (2001)
3 other articles

8 mentions without citation

Atomic-level protein structure refinement using fragment-guided molecular dynamics conformation sampling.
Zhang et al. (2011)
7 more