› 1dkp

X-ray diffraction
2.28Å resolution

CRYSTAL STRUCTURE OF PHYTATE COMPLEX OF ESCHERICHIA COLI PHYTASE AT PH 6.6. PHYTATE IS BOUND WITH ITS 3-PHOSPHATE IN THE ACTIVE SITE. HG2+ CATION ACTS AS AN INTERMOLECULAR BRIDGE

Released:
DOI: 10.2210/pdb1dkp/pdb
PDB entry 1dkp coloured by chain, front view.
PDB entry 1dkp coloured by chain, side view.
PDB entry 1dkp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of Escherichia coli phytase and its complex with phytate.
Lim D, Golovan S, Forsberg CW, Jia Z
Nat Struct Biol 7 108-13 (2000)
PMID: 10655611

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytase AppA Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 44.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07102 (Residues: 23-432; Coverage: 100%)
Gene names: JW0963, appA, b0980
Sequence domains: Histidine phosphatase superfamily (branch 2)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

2 bound ligands:
Ligand HG 4 x HG
Ligand IHP 1 x IHP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 70.573Å b: 75.127Å c: 90.393Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.227
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Production, purification and properties of microbial phytases.
Pandey et al. (2001)
4 more

7 mentions without citation

Avian multiple inositol polyphosphate phosphatase is an active phytase that can be engineered to help ameliorate the planet's "phosphate crisis".
Cho et al. (2006)
6 more