› 1dl3

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF MUTUALLY GENERATED MONOMERS OF DIMERIC PHOSPHORIBOSYLANTRANILATE ISOMERASE FROM THERMOTOGA MARITIMA

Released:
DOI: 10.2210/pdb1dl3/pdb
PDB entry 1dl3 coloured by chain, front view.
PDB entry 1dl3 coloured by chain, side view.
PDB entry 1dl3 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structure and function of mutationally generated monomers of dimeric phosphoribosylanthranilate isomerase from Thermotoga maritima.
Thoma R, Hennig M, Sterner R, Kirschner K
Structure 8 265-76 (2000)
PMID: 10745009

Function and Biology Details

Reaction catalysed: 
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-(5'-phosphoribosyl)anthranilate isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 203 amino acids
Theoretical weight: 22.97 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56320 (Residues: 1-205; Coverage: 99%)
Gene names: TM_0139, trpF
Sequence domains: N-(5'phosphoribosyl)anthranilate (PRA) isomerase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P21212
Unit cell:
a: 90.68Å b: 94.65Å c: 46.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.282
Expression system: Escherichia coli

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Citations

6 review citations

Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability.
Vieille et al. (2001)
5 more

1 mention without citation

De novo enzyme design using Rosetta3.
Richter et al. (2011)