› 1dll

X-ray diffraction
1.8Å resolution

The HC fragement of tetanus toxin complexed with lactose

Released:
DOI: 10.2210/pdb1dll/pdb
PDB entry 1dll coloured by chain, front view.
PDB entry 1dll coloured by chain, side view.
PDB entry 1dll coloured by chain, top view.
Source organism: Clostridium tetani
Primary publication:
The structures of the H(C) fragment of tetanus toxin with carbohydrate subunit complexes provide insight into ganglioside binding.
Emsley P, Fotinou C, Black I, Fairweather NF, Charles IG, Watts C, Hewitt E, Isaacs NW
J Biol Chem 275 8889-94 (2000)
PMID: 10722735

Function and Biology Details

Reaction catalysed: 
Hydrolysis of -Gln(76)-|-Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Tetanus toxin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 50.48 KDa
Source organism: Clostridium tetani
Expression system: Escherichia coli
UniProt:
  • Canonical: P04958 (Residues: 875-1315; Coverage: 34%)
Gene names: CTC_p60, tetX
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P212121
Unit cell:
a: 67.076Å b: 70.882Å c: 122.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.201 0.267
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Botulinum and tetanus neurotoxins: structure, function and therapeutic utility.
Turton et al. (2002)
16 more

6 mentions without citation

Tetanus Toxin Fragment C: Structure, Drug Discovery Research and Production.
Bayart et al. (2022)
5 more