› 1dma

X-ray diffraction
2.5Å resolution

DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH NICOTINAMIDE AND AMP

Released:
DOI: 10.2210/pdb1dma/pdb
PDB entry 1dma coloured by chain, front view.
PDB entry 1dma coloured by chain, side view.
PDB entry 1dma coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
The crystal structure of Pseudomonas aeruginosa exotoxin domain III with nicotinamide and AMP: conformational differences with the intact exotoxin.
Li M, Dyda F, Benhar I, Pastan I, Davies DR
Proc Natl Acad Sci U S A 92 9308-12 (1995)
PMID: 7568123

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exotoxin A Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 23.33 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P11439 (Residues: 425-638; Coverage: 35%)
Gene names: PA1148, eta
Sequence domains: Exotoxin A catalytic
Structure domains: Diphtheria Toxin, domain 1

Ligands and Environments

2 bound ligands:
Ligand NCA 2 x NCA
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 87.51Å b: 87.51Å c: 134.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.265
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Toward a unified nomenclature for mammalian ADP-ribosyltransferases.
Hottiger et al. (2010)
4 more

7 mentions without citation

Mesothelin-Targeted Recombinant Immunotoxins for Solid Tumors.
Hagerty et al. (2020)
6 more