› 1dmu

X-ray diffraction
2.2Å resolution

Crystal structure of the restriction endonuclease BglI (e.c.3.1.21.4) bound to its dna recognition sequence

Released:
DOI: 10.2210/pdb1dmu/pdb
PDB entry 1dmu coloured by chain, front view.
PDB entry 1dmu coloured by chain, side view.
PDB entry 1dmu coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of restriction endonuclease BglI bound to its interrupted DNA recognition sequence.
Newman M, Lunnen K, Wilson G, Greci J, Schildkraut I, Phillips SE
EMBO J. 17 5466-76 (1998)
PMID: 9736624

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113688 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme BglI Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.05 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O68557 (Residues: 1-299; Coverage: 100%)
Gene name: bglIR
Sequence domains: Restriction endonuclease BglI
Structure domains: Restriction endonuclease BglI
DNA (5'-D(*AP*TP*CP*GP*CP*CP*TP*AP*AP*TP*AP*GP*GP*CP*GP*AP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 17 nucleotides
Theoretical weight: 5.21 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 78.48Å b: 81.6Å c: 117.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.177 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
6 more

11 mentions without citation

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
10 more