› 1dmy

X-ray diffraction
2.45Å resolution

COMPLEX BETWEEN MURINE MITOCHONDRIAL CARBONIC ANYHDRASE V AND THE TRANSITION STATE ANALOGUE ACETAZOLAMIDE

Released:
DOI: 10.2210/pdb1dmy/pdb
PDB entry 1dmy coloured by chain, front view.
PDB entry 1dmy coloured by chain, side view.
PDB entry 1dmy coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure determination of murine mitochondrial carbonic anhydrase V at 2.45-A resolution: implications for catalytic proton transfer and inhibitor design.
Boriack-Sjodin PA, Heck RW, Laipis PJ, Silverman DN, Christianson DW
Proc Natl Acad Sci U S A 92 10949-53 (1995)
PMID: 7479916

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150021 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 5A, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 28.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P23589 (Residues: 53-299; Coverage: 83%)
Gene names: Ca5, Ca5a, Car5, Car5a
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand AZM 2 x AZM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 48.69Å b: 60.37Å c: 60.44Å
α: 67.65° β: 75.26° γ: 75.21°
R-values:
R R work R free
0.17 0.17 not available
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Voltage-gated proton channels and other proton transfer pathways.
Decoursey TE. (2003)
9 more

4 mentions without citation

Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding.
Krishnamurthy et al. (2008)
3 more