› 1dof

X-ray diffraction
2.1Å resolution

THE CRYSTAL STRUCTURE OF ADENYLOSUCCINATE LYASE FROM PYROBACULUM AEROPHILUM: INSIGHTS INTO THERMAL STABILITY AND HUMAN PATHOLOGY

Released:
DOI: 10.2210/pdb1dof/pdb
PDB entry 1dof coloured by chain, front view.
PDB entry 1dof coloured by chain, side view.
PDB entry 1dof coloured by chain, top view.
Source organism: Pyrobaculum aerophilum
Primary publication:
The crystal structure of adenylosuccinate lyase from Pyrobaculum aerophilum reveals an intracellular protein with three disulfide bonds.
Toth EA, Worby C, Dixon JE, Goedken ER, Marqusee S, Yeates TO
J Mol Biol 301 433-50 (2000)
PMID: 10926519

Function and Biology Details

Reaction catalysed: 
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 403 amino acids
Theoretical weight: 45.42 KDa
Source organism: Pyrobaculum aerophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZY28 (Residues: 1-403; Coverage: 100%)
Gene name: PAE0972
Sequence domains: Lyase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 65.62Å b: 150.31Å c: 173.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.245
Expression system: Escherichia coli

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Citations

11 review citations

Posttranslational protein modification in Archaea.
Eichler et al. (2005)
10 more

7 mentions without citation

Engineering by Cuts: How Kirigami Principle Enables Unique Mechanical Properties and Functionalities.
Tao et al. (2022)
6 more