› 1drt

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE IN COMPLEX WITH FE(II), 2-OXOGLUTARATE AND PROCLAVAMINIC ACID

Released:
DOI: 10.2210/pdb1drt/pdb
PDB entry 1drt coloured by chain, front view.
PDB entry 1drt coloured by chain, side view.
PDB entry 1drt coloured by chain, top view.
Source organism: Streptomyces clavuligerus
Primary publication:
Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase.
Zhang Z, Ren J, Stammers DK, Baldwin JE, Harlos K, Schofield CJ
Nat Struct Biol 7 127-33 (2000)
PMID: 10655615

Function and Biology Details

Reaction catalysed: 
Deoxyamidinoproclavaminate + 2-oxoglutarate + O(2) = amidinoproclavaminate + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170219 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Clavaminate synthase 1 Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 35.42 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05581 (Residues: 1-324; Coverage: 100%)
Gene name: cs1
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like

Ligands and Environments

3 bound ligands:
Ligand PCV 1 x PCV
Ligand FE2 1 x FE2
Ligand AKG 1 x AKG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 65.48Å b: 68.64Å c: 69.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.269
Expression system: Escherichia coli

Quick links

Citations

32 review citations

Oxygen sensing by HIF hydroxylases.
Schofield et al. (2004)
31 more

9 mentions without citation

Structural genomics: a pipeline for providing structures for the biologist.
Chance et al. (2002)
8 more