› 1dsy

X-ray diffraction
2.6Å resolution

C2 DOMAIN FROM PROTEIN KINASE C (ALPHA) COMPLEXED WITH CA2+ AND PHOSPHATIDYLSERINE

Released:
DOI: 10.2210/pdb1dsy/pdb
PDB entry 1dsy coloured by chain, front view.
PDB entry 1dsy coloured by chain, side view.
PDB entry 1dsy coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Ca(2+) bridges the C2 membrane-binding domain of protein kinase Calpha directly to phosphatidylserine.
Verdaguer N, Corbalan-Garcia S, Ochoa WF, Fita I, Gómez-Fernández JC
EMBO J 18 6329-38 (1999)
PMID: 10562545

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C alpha type Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 16.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05696 (Residues: 155-293; Coverage: 21%)
Gene names: Pkca, Prkca
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand PO4 1 x PO4
Ligand PSF 1 x PSF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3221
Unit cell:
a: 58.8Å b: 58.8Å c: 91.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.195 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

Membrane recognition by phospholipid-binding domains.
Lemmon MA. (2008)
34 more

37 mentions without citation

Conditional peripheral membrane proteins: facing up to limited specificity.
Moravcevic et al. (2012)
36 more