› 1dt0

X-ray diffraction
2.1Å resolution

CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS

Released:
DOI: 10.2210/pdb1dt0/pdb
PDB entry 1dt0 coloured by chain, front view.
PDB entry 1dt0 coloured by chain, side view.
PDB entry 1dt0 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis.
Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL
Acta Crystallogr D Biol Crystallogr 56 1359-66 (2000)
PMID: 11053832

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Fe] Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 197 amino acids
Theoretical weight: 21.91 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P09223 (Residues: 2-197; Coverage: 99%)
Gene name: sodB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FE 3 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3221
Unit cell:
a: 115.03Å b: 115.03Å c: 82.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.29
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Identification of a mitochondrial superoxide dismutase with an unusual targeting sequence in Plasmodium falciparum.
Sienkiewicz et al. (2004)
1 more

2 mentions without citation

Detection, distribution and characterization of novel superoxide dismutases from Yersinia enterocolitica Biovar 1A.
Dhar et al. (2013)
1 more