› 1dwf

X-ray diffraction
2Å resolution

Study on radiation damage on a cryocooled crystal. Part 2: Structure after irradiation with 9.1*10e15 photons/mm2

Released:
DOI: 10.2210/pdb1dwf/pdb
PDB entry 1dwf coloured by chain, front view.
PDB entry 1dwf coloured by chain, side view.
PDB entry 1dwf coloured by chain, top view.
Source organism: Sinapis alba
Primary publication:
Structural changes in a cryo-cooled protein crystal owing to radiation damage.
Burmeister WP
Acta Crystallogr D Biol Crystallogr 56 328-41 (2000)
PMID: 10713520

Function and Biology Details

Reaction catalysed: 
A thioglucoside + H(2)O = a sugar + a thiol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Myrosinase MA1 Chain: M
Molecule details ›
Chain: M
Length: 499 amino acids
Theoretical weight: 56.85 KDa
Source organism: Sinapis alba
UniProt:
  • Canonical: P29736 (Residues: 3-501; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, XYP, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, XYP, FUC, MAN
Carbohydrate polymer
4 bound ligands:
Ligand NAG 6 x NAG
Ligand ZN 1 x ZN
Ligand SO4 8 x SO4
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2221
Unit cell:
a: 134.3Å b: 136.4Å c: 80.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.18

Quick links

Citations

14 review citations

Channelrhodopsin unchained: structure and mechanism of a light-gated cation channel.
Lórenz-Fonfría et al. (2014)
13 more

4 mentions without citation

Molecular Modeling of Myrosinase from Brassica oleracea: A Structural Investigation of Sinigrin Interaction.
Natarajan et al. (2015)
3 more