› 1dx9

X-ray diffraction
2.05Å resolution

W57A Apoflavodoxin from Anabaena

Released:
DOI: 10.2210/pdb1dx9/pdb
PDB entry 1dx9 coloured by chain, front view.
PDB entry 1dx9 coloured by chain, side view.
PDB entry 1dx9 coloured by chain, top view.
Source organism: Nostoc sp. PCC 7119
Primary publication:
Dissecting the energetics of the apoflavodoxin-FMN complex.
Lostao A, El Harrous M, Daoudi F, Romero A, Parody-Morreale A, Sancho J
J Biol Chem 275 9518-26 (2000)
PMID: 10734100

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 169 amino acids
Theoretical weight: 18.73 KDa
Source organism: Nostoc sp. PCC 7119
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3E0 (Residues: 2-170; Coverage: 99%)
Gene name: isiB
Sequence domains: Flavodoxin
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P21
Unit cell:
a: 74.14Å b: 55.52Å c: 75.18Å
α: 90° β: 107.04° γ: 90°
R-values:
R R work R free
0.169 0.169 0.225
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Direct measurement of protein binding energetics by isothermal titration calorimetry.
Leavitt et al. (2001)
3 more

1 mention without citation

Defining the nature of thermal intermediate in 3 state folding proteins: apoflavodoxin, a study case.
García-Fandiño et al. (2012)