› 1dys

X-ray diffraction
1.6Å resolution

Endoglucanase CEL6B from Humicola insolens

Released:
DOI: 10.2210/pdb1dys/pdb
PDB entry 1dys coloured by chain, front view.
PDB entry 1dys coloured by chain, side view.
PDB entry 1dys coloured by chain, top view.
Source organism: Humicola insolens
Primary publication:
Structure and function of Humicola insolens family 6 cellulases: structure of the endoglucanase, Cel6B, at 1.6 A resolution.
Davies GJ, Brzozowski AM, Dauter M, Varrot A, Schülein M
Biochem J 348 Pt 1 201-7 (2000)
PMID: 10794732

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase-6B Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 37.79 KDa
Source organism: Humicola insolens
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: Q7SIG5 (Residues: 1-348; Coverage: 100%)
Gene name: cel6B
Sequence domains: Glycosyl hydrolases family 6
Structure domains: 1, 4-beta cellobiohydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21212
Unit cell:
a: 109.917Å b: 104.451Å c: 53.788Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.24
Expression system: Aspergillus oryzae

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Citations

1 review citation

Glycoside hydrolases: catalytic base/nucleophile diversity.
Vuong et al. (2010)
23 other articles

9 mentions without citation

Genomic insights into the fungal lignocellulolytic system of Myceliophthora thermophila.
Karnaouri et al. (2014)
8 more