› 1dzb

X-ray diffraction
2Å resolution

Crystal structure of phage library-derived single-chain Fv fragment 1F9 in complex with turkey egg-white lysozyme

Released:
DOI: 10.2210/pdb1dzb/pdb
PDB entry 1dzb coloured by chain, front view.
PDB entry 1dzb coloured by chain, side view.
PDB entry 1dzb coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a phage library-derived single-chain Fv fragment complexed with turkey egg-white lysozyme at 2.0 A resolution.
Aÿ J, Keitel T, Küttner G, Wessner H, Scholz C, Hahn M, Höhne W
J Mol Biol 301 239-46 (2000)
PMID: 10926506

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208285 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SCFV FRAGMENT 1F9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 27.25 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Lysozyme C Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 129 amino acids
Theoretical weight: 14.23 KDa
Source organism: Meleagris gallopavo
UniProt:
  • Canonical: P00703 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P21
Unit cell:
a: 38.71Å b: 112.44Å c: 80Å
α: 90° β: 97.67° γ: 90°
R-values:
R R work R free
0.225 0.225 0.306

Quick links

Citations

18 citation in other articles

Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies.
De Genst et al. (2006)
17 more

10 mentions without citation

Structural basis of neutralization by a human anti-severe acute respiratory syndrome spike protein antibody, 80R.
Hwang et al. (2006)
9 more