› 1e12

X-ray diffraction
1.8Å resolution

Halorhodopsin, a light-driven chloride pump

Released:
DOI: 10.2210/pdb1e12/pdb
PDB entry 1e12 coloured by chain, front view.
PDB entry 1e12 coloured by chain, side view.
PDB entry 1e12 coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Structure of the light-driven chloride pump halorhodopsin at 1.8 A resolution.
Kolbe M, Besir H, Essen LO, Oesterhelt D
Science 288 1390-6 (2000)
PMID: 10827943

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Halorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 26.95 KDa
Source organism: Halobacterium salinarum
UniProt:
  • Canonical: P0DMH7 (Residues: 22-274; Coverage: 92%)
Gene names: VNG_0180G, hop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

5 bound ligands:
Ligand CL 1 x CL
Ligand K 1 x K
Ligand PLM 1 x PLM
Ligand OLC 10 x OLC
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P6322
Unit cell:
a: 67.3Å b: 67.3Å c: 209.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.237 0.257

Quick links

Citations

64 review citations

Targeting and readout strategies for fast optical neural control in vitro and in vivo.
Gradinaru et al. (2007)
63 more

53 mentions without citation

Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.
Ernst et al. (2014)
52 more