› 1e1m

X-ray diffraction
1.85Å resolution

ADRENODOXIN REDUCTASE in complex with NADPH obtained by a soaking experiment

Released:
DOI: 10.2210/pdb1e1m/pdb
PDB entry 1e1m coloured by chain, front view.
PDB entry 1e1m coloured by chain, side view.
PDB entry 1e1m coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structures of adrenodoxin reductase in complex with NADP+ and NADPH suggesting a mechanism for the electron transfer of an enzyme family.
Ziegler GA, Schulz GE
Biochemistry 39 10986-95 (2000)
PMID: 10998235

Function and Biology Details

Reaction catalysed: 
2 reduced adrenodoxin + NADP(+) = 2 oxidized adrenodoxin + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH:adrenodoxin oxidoreductase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 50.36 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08165 (Residues: 33-492; Coverage: 94%)
Gene names: ADXR, FDXR
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 61.5Å b: 62.5Å c: 78.6Å
α: 90° β: 106.7° γ: 90°
R-values:
R R work R free
0.188 0.188 0.228
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Variations on a (t)heme--novel mechanisms, redox partners and catalytic functions in the cytochrome P450 superfamily.
Munro et al. (2007)
8 more

2 mentions without citation

A structurally conserved water molecule in Rossmann dinucleotide-binding domains.
Bottoms et al. (2002)
1 more