› 1e3m

X-ray diffraction
2.2Å resolution

The crystal structure of E. coli MutS binding to DNA with a G:T mismatch

Released:
DOI: 10.2210/pdb1e3m/pdb
PDB entry 1e3m coloured by chain, front view.
PDB entry 1e3m coloured by chain, side view.
PDB entry 1e3m coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of DNA mismatch repair protein MutS binding to a G x T mismatch.
Lamers MH, Perrakis A, Enzlin JH, Winterwerp HH, de Wind N, Sixma TK
Nature 407 711-7 (2000)
PMID: 11048711

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113718 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 800 amino acids
Theoretical weight: 90.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P23909 (Residues: 1-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:
Structure domains:
5'-D(*AP*GP*CP*TP*GP*CP*CP*AP*GP*GP*CP*AP*CP*CP*AP* GP*TP*GP*TP*CP*AP*GP*CP*GP*TP*CP*CP*TP*AP*T)-3' Chain: E
Molecule details ›
Chain: E
Length: 30 nucleotides
Theoretical weight: 9.18 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*AP*TP*AP*GP*GP*AP*CP*GP*CP*TP*GP*AP*CP*AP*CP* TP*GP*GP*TP*GP*CP*TP*TP*GP*GP*CP*AP*GP*CP*T)-3' Chain: F
Molecule details ›
Chain: F
Length: 30 nucleotides
Theoretical weight: 9.28 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 89.96Å b: 92.37Å c: 261.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.266
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

90 review citations

Genome maintenance mechanisms for preventing cancer.
Hoeijmakers JH. (2001)
89 more

35 mentions without citation

Single-molecule views of MutS on mismatched DNA.
Lee et al. (2014)
34 more