› 1e44

X-ray diffraction
2.4Å resolution

ribonuclease domain of colicin E3 in complex with its immunity protein

Released:
DOI: 10.2210/pdb1e44/pdb
PDB entry 1e44 coloured by chain, front view.
PDB entry 1e44 coloured by chain, side view.
PDB entry 1e44 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Inhibition of a ribosome-inactivating ribonuclease: the crystal structure of the cytotoxic domain of colicin E3 in complex with its immunity protein.
Carr S, Walker D, James R, Kleanthous C, Hemmings AM
Structure 8 949-60 (2000)
PMID: 10986462

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132796 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin E3 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02984 (Residues: 1-85; Coverage: 100%)
Gene names: ceiC, imm, immB
Sequence domains: Cloacin immunity protein
Structure domains: Cloacin immunity protein
Colicin E3 Chain: B
Molecule details ›
Chain: B
Length: 96 amino acids
Theoretical weight: 10.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00646 (Residues: 456-551; Coverage: 17%)
Gene names: ceaC, col
Sequence domains: Cytotoxic
Structure domains: Colicin E3-like ribonuclease domain

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P3121
Unit cell:
a: 93.7Å b: 93.7Å c: 76.17Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli BL21

Quick links

Citations

8 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
7 more

10 mentions without citation

Colicin biology.
Cascales et al. (2007)
9 more