› 1e4d

X-ray diffraction
1.8Å resolution

Structure of OXA10 beta-lactamase at pH 8.3

Released:
DOI: 10.2210/pdb1e4d/pdb
PDB entry 1e4d coloured by chain, front view.
PDB entry 1e4d coloured by chain, side view.
PDB entry 1e4d coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Insights into class D beta-lactamases are revealed by the crystal structure of the OXA10 enzyme from Pseudomonas aeruginosa.
Maveyraud L, Golemi D, Kotra LP, Tranier S, Vakulenko S, Mobashery S, Samama JP
Structure 8 1289-98 (2000)
PMID: 11188693

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase OXA-10 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 246 amino acids
Theoretical weight: 27.57 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14489 (Residues: 21-266; Coverage: 100%)
Gene names: bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand SO4 16 x SO4
Ligand EDO 6 x EDO
1 modified residue:
Ligand KCX 4 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 67.279Å b: 82.436Å c: 101.165Å
α: 90° β: 95.89° γ: 90°
R-values:
R R work R free
0.198 0.198 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
13 more

2 mentions without citation

Class D β-lactamases: a reappraisal after five decades.
Leonard et al. (2013)
1 more