› 1e4w

X-ray diffraction
1.95Å resolution

crossreactive binding of a circularized peptide to an anti-TGFalpha antibody Fab-fragment

Released:
DOI: 10.2210/pdb1e4w/pdb
PDB entry 1e4w coloured by chain, front view.
PDB entry 1e4w coloured by chain, side view.
PDB entry 1e4w coloured by chain, top view.
Source organisms:
Primary publication:
Cross-reactive binding of cyclic peptides to an anti-TGFalpha antibody Fab fragment: an X-ray structural and thermodynamic analysis.
Hahn M, Winkler D, Welfle K, Misselwitz R, Welfle H, Wessner H, Zahn G, Scholz C, Seifert M, Harkins R, Schneider-Mergener J, Höhne W
J Mol Biol 314 293-309 (2001)
PMID: 11718562

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134668 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig gamma-2A chain C region, membrane-bound form Chain: H
Molecule details ›
Chain: H
Length: 213 amino acids
Theoretical weight: 22.88 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01865 (Residues: 1-98; Coverage: 25%)
Gene name: Igh-1a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-107; Coverage: 100%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
CYCLIC PEPTIDE Chain: P
Molecule details ›
Chain: P
Length: 7 amino acids
Theoretical weight: 926 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 49.22Å b: 94.35Å c: 121.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.269
Expression system: Not provided

Quick links

Citations

4 review citations

Peptide arrays: from macro to micro.
Reimer et al. (2002)
3 more

1 mention without citation

OptMAVEn--a new framework for the de novo design of antibody variable region models targeting specific antigen epitopes.
Li et al. (2014)