› 1e4y

X-ray diffraction
1.85Å resolution

Mutant P9L of adenylate kinase from E. coli, modified in the Gly-loop

Released:
DOI: 10.2210/pdb1e4y/pdb
PDB entry 1e4y coloured by chain, front view.
PDB entry 1e4y coloured by chain, side view.
PDB entry 1e4y coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of two mutants of adenylate kinase from Escherichia coli that modify the Gly-loop.
Müller CW, Schulz GE
Proteins 15 42-9 (1993)
PMID: 8451239

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 23.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69441 (Residues: 1-214; Coverage: 100%)
Gene names: JW0463, adk, b0474, dnaW, plsA
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand AP5 2 x AP5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 60.3Å b: 77.8Å c: 57.7Å
α: 90° β: 94.3° γ: 90°
R-values:
R R work R free
0.178 0.178 not available
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein conformational substates from X-ray crystallography.
Rejto et al. (1996)
19 other articles

11 mentions without citation

Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies.
Olenginski et al. (2021)
10 more