1e5h

X-ray diffraction
1.96Å resolution

DELTA-R307A DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE AND CARBON DIOXIDE

Released:

Function and Biology Details

Reaction catalysed:
Penicillin N + 2-oxoglutarate + O(2) = deacetoxycephalosporin C + succinate + CO(2) + H(2)O
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
PDBe Complex ID:
PDB-CPX-148404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deacetoxycephalosporin C synthase Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 34.27 KDa
Source organism: Streptomyces clavuligerus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18548 (Residues: 1-308; Coverage: 99%)
Gene name: cefE
Sequence domains: 2OG-Fe(II) oxygenase superfamily
Structure domains: B-lactam Antibiotic, Isopenicillin N Synthase; Chain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: R3
Unit cell:
a: 106.26Å b: 106.26Å c: 71.06Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.252
Expression system: Escherichia coli BL21(DE3)