› 1e5x

X-ray diffraction
2.25Å resolution

Structure of threonine synthase from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb1e5x/pdb
PDB entry 1e5x coloured by chain, front view.
PDB entry 1e5x coloured by chain, side view.
PDB entry 1e5x coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structure of threonine synthase from Arabidopsis thaliana.
Thomazeau K, Curien G, Dumas R, Biou V
Protein Sci 10 638-48 (2001)
PMID: 11344332

Function and Biology Details

Reaction catalysed: 
O-phospho-L-homoserine + H(2)O = L-threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine synthase 1, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 486 amino acids
Theoretical weight: 54.06 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9S7B5 (Residues: 41-526; Coverage: 92%)
Gene names: At4g29840, F27B13.80, MTO2, TS1
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 26 x MSE
Ligand MSO 2 x MSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P1
Unit cell:
a: 57.76Å b: 62.14Å c: 76.59Å
α: 109.48° β: 97.61° γ: 112.74°
R-values:
R R work R free
0.222 0.222 0.243
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Interdependence of threonine, methionine and isoleucine metabolism in plants: accumulation and transcriptional regulation under abiotic stress.
Joshi et al. (2010)
5 more

8 mentions without citation

Structure-Based Statistical Mechanical Model Accounts for the Causality and Energetics of Allosteric Communication.
Guarnera et al. (2016)
7 more