› 1e6n

X-ray diffraction
2.25Å resolution

Chitinase B from Serratia marcescens inactive mutant E144Q in complex with N-acetylglucosamine-pentamer

Released:
DOI: 10.2210/pdb1e6n/pdb
PDB entry 1e6n coloured by chain, front view.
PDB entry 1e6n coloured by chain, side view.
PDB entry 1e6n coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Structural insights into the catalytic mechanism of a family 18 exo-chitinase.
van Aalten DM, Komander D, Synstad B, Gåseidnes S, Peter MG, Eijsink VG
Proc Natl Acad Sci U S A 98 8979-84 (2001)
PMID: 11481469

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145920 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitinase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 55.52 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11797 (Residues: 1-499; Coverage: 100%)
Gene name: chiB
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand SO4 12 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 55.732Å b: 104.476Å c: 186.676Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.239
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Lysozymes in the animal kingdom.
Callewaert et al. (2010)
21 more

14 mentions without citation

Structural insights into the catalytic mechanism of a family 18 exo-chitinase.
van Aalten et al. (2001)
13 more