› 1e7m

X-ray diffraction
2.54Å resolution

ACTIVE SITE MUTANT (D177->N) OF GLUCOSE 6-PHOSPHATE DEHYDROGENASE FROM LEUCONOSTOC MESENTEROIDES

Released:
DOI: 10.2210/pdb1e7m/pdb
PDB entry 1e7m coloured by chain, front view.
PDB entry 1e7m coloured by chain, side view.
PDB entry 1e7m coloured by chain, top view.
Source organism: Leuconostoc mesenteroides
Primary publication:
An examination of the role of asp-177 in the His-Asp catalytic dyad of Leuconostoc mesenteroides glucose 6-phosphate dehydrogenase: X-ray structure and pH dependence of kinetic parameters of the D177N mutant enzyme.
Cosgrove MS, Gover S, Naylor CE, Vandeputte-Rutten L, Adams MJ, Levy HR
Biochemistry 39 15002-11 (2000)
PMID: 11106478

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate + NAD(P)(+) = 6-phospho-D-glucono-1,5-lactone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate 1-dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 54.37 KDa
Source organism: Leuconostoc mesenteroides
Expression system: Escherichia coli
UniProt:
  • Canonical: P11411 (Residues: 2-486; Coverage: 100%)
Gene name: zwf
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 130.3Å b: 44.4Å c: 92.8Å
α: 90° β: 106.7° γ: 90°
R-values:
R R work R free
0.222 0.222 0.299
Expression system: Escherichia coli

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Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
20 other articles