› 1e85

X-ray diffraction
1.35Å resolution

Cytochrome c' from Alcaligenes xylosoxidans - reduced structure with NO bound to proximal side of heme

Released:
DOI: 10.2210/pdb1e85/pdb
PDB entry 1e85 coloured by chain, front view.
PDB entry 1e85 coloured by chain, side view.
PDB entry 1e85 coloured by chain, top view.
Source organism: Achromobacter xylosoxidans
Primary publication:
Unprecedented proximal binding of nitric oxide to heme: implications for guanylate cyclase.
Lawson DM, Stevenson CE, Andrew CR, Eady RR
EMBO J 19 5661-71 (2000)
PMID: 11060017

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c' Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.63 KDa
Source organism: Achromobacter xylosoxidans
UniProt:
  • Canonical: P00138 (Residues: 1-127; Coverage: 100%)
Sequence domains: Cytochrome C'
Structure domains: Cytochrome c/b562

Ligands and Environments

2 bound ligands:
Ligand HEC 1 x HEC
Ligand NO 1 x NO
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P6522
Unit cell:
a: 53.035Å b: 53.035Å c: 180.993Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.194 0.22

Quick links

Citations

15 review citations

Structure and regulation of soluble guanylate cyclase.
Derbyshire et al. (2012)
14 more

4 mentions without citation

The crystal structure of cytochrome P460 of Nitrosomonas europaea reveals a novel cytochrome fold and heme-protein cross-link.
Pearson et al. (2007)
3 more