› 1e8n

X-ray diffraction
1.5Å resolution

PROLYL OLIGOPEPTIDASE FROM PORCINE BRAIN, MUTANT, COMPLEXED WITH PEPTIDE

Released:
DOI: 10.2210/pdb1e8n/pdb
PDB entry 1e8n coloured by chain, front view.
PDB entry 1e8n coloured by chain, side view.
PDB entry 1e8n coloured by chain, top view.
Source organisms:
Primary publication:
Structures of prolyl oligopeptidase substrate/inhibitor complexes. Use of inhibitor binding for titration of the catalytic histidine residue.
Fülöp V, Szeltner Z, Renner V, Polgár L
J Biol Chem 276 1262-6 (2001)
PMID: 11031266

Function and Biology Details

Reaction catalysed: 
Hydrolysis of --Pro-|- and to a lesser extent --Ala-|- in oligopeptides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150043 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Prolyl endopeptidase Chain: A
Molecule details ›
Chain: A
Length: 710 amino acids
Theoretical weight: 80.85 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P23687 (Residues: 1-710; Coverage: 100%)
Gene name: PREP
Sequence domains:
Structure domains:
PEPTIDE INHIBITOR Chain: I
Molecule details ›
Chain: I
Length: 9 amino acids
Theoretical weight: 918 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
1 modified residue:
Ligand BE2 1 x BE2

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 70.7Å b: 99.7Å c: 110.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.212
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Dipeptidyl-peptidase IV from bench to bedside: an update on structural properties, functions, and clinical aspects of the enzyme DPP IV.
Lambeir et al. (2003)
3 more

7 mentions without citation

The overlap of small molecule and protein binding sites within families of protein structures.
Davis et al. (2010)
6 more