› 1e8r

Solution NMR

SOLUTION STRUCTURE OF TYPE X CBD

Released:
DOI: 10.2210/pdb1e8r/pdb
PDB entry 1e8r coloured by chain, ensemble of 5 models, front view.
PDB entry 1e8r coloured by chain, ensemble of 5 models, side view.
PDB entry 1e8r coloured by chain, ensemble of 5 models, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Solution structure of the CBM10 cellulose binding module from Pseudomonas xylanase A.
Raghothama S, Simpson PJ, Szabó L, Nagy T, Gilbert HJ, Williamson MP
Biochemistry 39 978-84 (2000)
PMID: 10653641

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase A Chain: A
Molecule details ›
Chain: A
Length: 50 amino acids
Theoretical weight: 5.4 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14768 (Residues: 180-228; Coverage: 8%)
Gene names: CJA_2471, xyn10A, xynA
Sequence domains: Cellulose or protein binding domain
Structure domains: CBM10

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY, SIMULATED ANNEALING
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.
Fontes et al. (2010)
3 more

6 mentions without citation

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
5 more