› 1e97

X-ray diffraction
2Å resolution

Crystal structure of ketosteroid isomerase from Pseudomonas putida ; triple mutant y16f/y32f/y57f

Released:
DOI: 10.2210/pdb1e97/pdb
PDB entry 1e97 coloured by chain, front view.
PDB entry 1e97 coloured by chain, side view.
PDB entry 1e97 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Pseudoreversion of the catalytic activity of Y14F by the additional substitution(s) of tyrosine with phenylalanine in the hydrogen bond network of delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.
Choi G, Ha NC, Kim MS, Hong BH, Oh BH, Choi KY
Biochemistry 40 6828-35 (2001)
PMID: 11389596

Function and Biology Details

Reaction catalysed: 
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.5 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P07445 (Residues: 1-131; Coverage: 100%)
Gene name: ksi
Sequence domains: SnoaL-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 36.33Å b: 96.53Å c: 74.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.264
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

Extreme electric fields power catalysis in the active site of ketosteroid isomerase.
Fried et al. (2014)
12 more

2 mentions without citation

Rescue of deleterious mutations by the compensatory Y30F mutation in ketosteroid isomerase.
Cha et al. (2013)
1 more