› 1e9f

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + dTMP = ADP + dTDP
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate kinase Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.22 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P23919 (Residues: 1-15, 20-144, 149-212; Coverage: 96%)
  • Canonical: P0A720 (Residues: 13-16, 151-156; Coverage: 5%)
Gene names: CDC8, DTYMK, JW1084, TMPK, TYMK, b1098, tmk, ycfG
Sequence domains: Thymidylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand TMP 1 x TMP
Ligand ADP 1 x ADP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P43212
Unit cell:
a: 100.2Å b: 100.2Å c: 49.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.283
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A roadmap to safe, efficient, and stable lentivirus-mediated gene therapy with hematopoietic cell transplantation.
Neschadim et al. (2007)
2 more

1 mention without citation

PDBe: Protein Data Bank in Europe.
Velankar et al. (2010)