› 1e9y

X-ray diffraction
3Å resolution

Crystal structure of Helicobacter pylori urease in complex with acetohydroxamic acid

Released:
DOI: 10.2210/pdb1e9y/pdb
PDB entry 1e9y coloured by chain, front view.
PDB entry 1e9y coloured by chain, side view.
PDB entry 1e9y coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Supramolecular assembly and acid resistance of Helicobacter pylori urease.
Ha NC, Oh ST, Sung JY, Cha KA, Lee MH, Oh BH
Nat Struct Biol 8 505-9 (2001)
PMID: 11373617

Function and Biology Details

Reaction catalysed: 
Urea + H(2)O = CO(2) + 2 NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147152 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urease subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 26.57 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: P14916 (Residues: 1-238; Coverage: 100%)
Gene names: HP_0073, hpuA, ureA
Sequence domains:
Structure domains:
Urease subunit beta Chain: B
Molecule details ›
Chain: B
Length: 569 amino acids
Theoretical weight: 61.76 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: P69996 (Residues: 1-569; Coverage: 100%)
Gene names: HP_0072, hpuB, ureB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand HAE 1 x HAE
Ligand NI 2 x NI
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: I23
Unit cell:
a: 178.16Å b: 178.16Å c: 178.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.282
Expression system: Escherichia coli

Quick links

Citations

36 review citations

Pathogenesis of Helicobacter pylori infection.
Kusters et al. (2006)
35 more

32 mentions without citation

Nonredox nickel enzymes.
Maroney et al. (2014)
31 more