› 1eae

X-ray diffraction
2.6Å resolution

ATOMIC STRUCTURE OF THE CUBIC CORE OF THE PYRUVATE DEHYDROGENASE MULTIENZYME COMPLEX

Released:
DOI: 10.2210/pdb1eae/pdb
PDB entry 1eae coloured by chain, front view.
PDB entry 1eae coloured by chain, side view.
PDB entry 1eae coloured by chain, top view.
Source organism: Azotobacter vinelandii
Primary publication:
Crystallographic analysis of substrate binding and catalysis in dihydrolipoyl transacetylase (E2p).
Mattevi A, Obmolova G, Kalk KH, Teplyakov A, Hol WG
Biochemistry 32 3887-901 (1993)
PMID: 8471601

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 26.24 KDa
Source organism: Azotobacter vinelandii
Expression system: Not provided
UniProt:
  • Canonical: P10802 (Residues: 396-638; Coverage: 38%)
Sequence domains: 2-oxoacid dehydrogenases acyltransferase (catalytic domain)
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

1 bound ligand:
Ligand LPM 1 x LPM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: F432
Unit cell:
a: 225.54Å b: 225.54Å c: 225.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 not available
Expression system: Not provided

Quick links

Citations

8 review citations

Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions.
Perham RN. (2000)
7 more

7 mentions without citation

An improved model of the Aspergillus fumigatus CYP51A protein.
Fraczek et al. (2011)
6 more