› 1ecb

X-ray diffraction
2.7Å resolution

ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 GMP, 1 MG PER SUBUNIT

Released:
DOI: 10.2210/pdb1ecb/pdb
PDB entry 1ecb coloured by chain, front view.
PDB entry 1ecb coloured by chain, side view.
PDB entry 1ecb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site.
Krahn JM, Kim JH, Burns MR, Parry RJ, Zalkin H, Smith JL
Biochemistry 36 11061-8 (1997)
PMID: 9333323

Function and Biology Details

Reaction catalysed: 
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-142776 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidophosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 504 amino acids
Theoretical weight: 56.42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG16 (Residues: 2-505; Coverage: 100%)
Gene names: JW2309, b2312, purF
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand 5GP 8 x 5GP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 95.8Å b: 113.2Å c: 199.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Channeling of substrates and intermediates in enzyme-catalyzed reactions.
Huang et al. (2001)
20 more

4 mentions without citation

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
3 more