› 1ecc

X-ray diffraction
2.4Å resolution

ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH MN-CPRPP AND 5-OXO-NORLEUCINE

Released:
DOI: 10.2210/pdb1ecc/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1ecc coloured by chain, front view.
PDB entry 1ecc coloured by chain, side view.
PDB entry 1ecc coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site.
Krahn JM, Kim JH, Burns MR, Parry RJ, Zalkin H, Smith JL
Biochemistry 36 11061-8 (1997)
PMID: 9333323

Function and Biology Details

Reaction catalysed: 
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidophosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 504 amino acids
Theoretical weight: 56.42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG16 (Residues: 2-505; Coverage: 100%)
Gene names: JW2309, b2312, purF
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 5 x MN
Ligand ONL 2 x ONL
Ligand PCP 2 x PCP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RUH2R
Spacegroup: P43212
Unit cell:
a: 78.2Å b: 78.2Å c: 308.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Channeling of substrates and intermediates in enzyme-catalyzed reactions.
Huang et al. (2001)
20 more

6 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
5 more