› 1ecf

X-ray diffraction
2Å resolution

ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE

Released:
DOI: 10.2210/pdb1ecf/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1ecf coloured by chain, front view.
PDB entry 1ecf coloured by chain, side view.
PDB entry 1ecf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli.
Muchmore CR, Krahn JM, Kim JH, Zalkin H, Smith JL
Protein Sci 7 39-51 (1998)
PMID: 9514258

Function and Biology Details

Reaction catalysed: 
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidophosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 504 amino acids
Theoretical weight: 56.42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG16 (Residues: 2-505; Coverage: 100%)
Gene names: JW2309, b2312, purF
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PIN 6 x PIN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RUH2R
Spacegroup: C2221
Unit cell:
a: 116.9Å b: 157.5Å c: 106.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Channeling of substrates and intermediates in enzyme-catalyzed reactions.
Huang et al. (2001)
4 more

8 mentions without citation

Decision-making in structure solution using Bayesian estimates of map quality: the PHENIX AutoSol wizard.
Terwilliger et al. (2009)
7 more