› 1ecj

X-ray diffraction
2.5Å resolution

ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 AMP PER TETRAMER

Released:
DOI: 10.2210/pdb1ecj/pdb
PDB entry 1ecj coloured by chain, front view.
PDB entry 1ecj coloured by chain, side view.
PDB entry 1ecj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli.
Muchmore CR, Krahn JM, Kim JH, Zalkin H, Smith JL
Protein Sci 7 39-51 (1998)
PMID: 9514258

Function and Biology Details

Reaction catalysed: 
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-142776 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidophosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 504 amino acids
Theoretical weight: 56.42 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AG16 (Residues: 2-505; Coverage: 100%)
Gene names: JW2309, b2312, purF
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 116.8Å b: 156Å c: 106.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Channeling of substrates and intermediates in enzyme-catalyzed reactions.
Huang et al. (2001)
4 more

4 mentions without citation

Affinity-based capture and identification of protein effectors of the growth regulator ppGpp.
Wang et al. (2019)
3 more