› 1ecy

X-ray diffraction
2.19Å resolution

PROTEASE INHIBITOR ECOTIN

Released:
DOI: 10.2210/pdb1ecy/pdb
PDB entry 1ecy coloured by chain, front view.
PDB entry 1ecy coloured by chain, side view.
PDB entry 1ecy coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure analyses of uncomplexed ecotin in two crystal forms: implications for its function and stability.
Shin DH, Song HK, Seong IS, Lee CS, Chung CH, Suh SW
Protein Sci 5 2236-47 (1996)
PMID: 8931142

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150067 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Ecotin Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23827 (Residues: 21-162; Coverage: 100%)
Gene names: JW2197, b2209, eco, eti
Sequence domains: Ecotin
Structure domains: Ecotin

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand GLC 4 x GLC
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P43212
Unit cell:
a: 63.32Å b: 63.32Å c: 84.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.319
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Designed Metal-ATCUN Derivatives: Redox- and Non-redox-Based Applications Relevant for Chemistry, Biology, and Medicine.
Maiti et al. (2020)
1 more

3 mentions without citation

Prokaryote-derived protein inhibitors of peptidases: A sketchy occurrence and mostly unknown function.
Kantyka et al. (2010)
2 more