› 1edo

X-ray diffraction
2.3Å resolution

THE X-RAY STRUCTURE OF BETA-KETO ACYL CARRIER PROTEIN REDUCTASE FROM BRASSICA NAPUS COMPLEXED WITH NADP+

Released:
DOI: 10.2210/pdb1edo/pdb
PDB entry 1edo coloured by chain, front view.
PDB entry 1edo coloured by chain, side view.
PDB entry 1edo coloured by chain, top view.
Source organism: Brassica napus
Primary publication:
The X-ray structure of Brassica napus beta-keto acyl carrier protein reductase and its implications for substrate binding and catalysis.
Fisher M, Kroon JT, Martindale W, Stuitje AR, Slabas AR, Rafferty JB
Structure 8 339-47 (2000)
PMID: 10801480

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase 1, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 25.36 KDa
Source organism: Brassica napus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93X62 (Residues: 77-320; Coverage: 76%)
Gene name: gbkr1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6422
Unit cell:
a: 129.28Å b: 129.28Å c: 92.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 0.235
Expression system: Escherichia coli

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Citations

9 review citations

The structural biology of type II fatty acid biosynthesis.
White et al. (2005)
8 more

10 mentions without citation

Structural analysis of actinorhodin polyketide ketoreductase: cofactor binding and substrate specificity.
Korman et al. (2004)
9 more