› 1eej

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE PROTEIN DISULFIDE BOND ISOMERASE, DSBC, FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb1eej/pdb
PDB entry 1eej coloured by chain, front view.
PDB entry 1eej coloured by chain, side view.
PDB entry 1eej coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli.
McCarthy AA, Haebel PW, Törrönen A, Rybin V, Baker EN, Metcalf P
Nat Struct Biol 7 196-9 (2000)
PMID: 10700276

Function and Biology Details

Reaction catalysed: 
Catalyzes the rearrangement of -S-S- bonds in proteins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol:disulfide interchange protein DsbC Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.49 KDa
Source organism: Escherichia coli
Expression system: Bacteria
UniProt:
  • Canonical: P0AEG6 (Residues: 21-236; Coverage: 100%)
Gene names: JW2861, b2893, dsbC, xprA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 59.336Å b: 78.149Å c: 94.489Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.247
Expression system: Bacteria

Quick links

Citations

52 review citations

Recombinant protein folding and misfolding in Escherichia coli.
Baneyx et al. (2004)
51 more

18 mentions without citation

Mechanisms of oxidative protein folding in the bacterial cell envelope.
Kadokura et al. (2010)
17 more