› 1eeo

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH ACETYL-E-L-E-F-PTYR-M-D-Y-E-NH2

Released:
DOI: 10.2210/pdb1eeo/pdb
PDB entry 1eeo coloured by chain, front view.
PDB entry 1eeo coloured by chain, side view.
PDB entry 1eeo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of plasticity in protein tyrosine phosphatase 1B substrate recognition.
Sarmiento M, Puius YA, Vetter SW, Keng YF, Wu L, Zhao Y, Lawrence DS, Almo SC, Zhang ZY
Biochemistry 39 8171-9 (2000)
PMID: 10889023

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148262 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 37.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
ACETYL-E-L-E-F-PTYR-M-D-Y-E-NH2 PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.34 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P212121
Unit cell:
a: 52.581Å b: 82.05Å c: 88.543Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.212
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

14 review citations

Structural and evolutionary relationships among protein tyrosine phosphatase domains.
Andersen et al. (2001)
13 more

9 mentions without citation

Insights into the reaction of protein-tyrosine phosphatase 1B: crystal structures for transition state analogs of both catalytic steps.
Brandão et al. (2010)
8 more