› 1ef4

Solution NMR

SOLUTION STRUCTURE OF THE ESSENTIAL RNA POLYMERASE SUBUNIT RPB10 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM

Released:
DOI: 10.2210/pdb1ef4/pdb
PDB entry 1ef4 coloured by chain, ensemble of 20 models, front view.
PDB entry 1ef4 coloured by chain, ensemble of 20 models, side view.
PDB entry 1ef4 coloured by chain, ensemble of 20 models, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum.
Mackereth CD, Arrowsmith CH, Edwards AM, McIntosh LP
Proc Natl Acad Sci U S A 97 6316-21 (2000)
PMID: 10841539

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-directed RNA polymerase subunit Rpo10 Chain: A
Molecule details ›
Chain: A
Length: 55 amino acids
Theoretical weight: 6.44 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O26147 (Residues: 1-55; Coverage: 100%)
Gene names: MTH_40, rpo10, rpoN
Sequence domains: RNA polymerases N / 8 kDa subunit
Structure domains: Homeodomain-like

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DISTANCE GEOMETRY WITH SIMULATED ANNEALING
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Zinc finger proteins: new insights into structural and functional diversity.
Laity et al. (2001)
6 more

5 mentions without citation

Structural classification of zinc fingers: survey and summary.
Krishna et al. (2003)
4 more