› 1eg7

X-ray diffraction
2.5Å resolution

THE CRYSTAL STRUCTURE OF FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA

Released:
DOI: 10.2210/pdb1eg7/pdb
PDB entry 1eg7 coloured by chain, front view.
PDB entry 1eg7 coloured by chain, side view.
PDB entry 1eg7 coloured by chain, top view.
Source organism: Moorella thermoacetica
Primary publication:
The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica.
Radfar R, Shin R, Sheldrick GM, Minor W, Lovell CR, Odom JD, Dunlap RB, Lebioda L
Biochemistry 39 3920-6 (2000)
PMID: 10747779

Function and Biology Details

Reaction catalysed: 
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate--tetrahydrofolate ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 557 amino acids
Theoretical weight: 59.87 KDa
Source organism: Moorella thermoacetica
Expression system: Escherichia coli
UniProt:
  • Canonical: P21164 (Residues: 1-559; Coverage: 100%)
Gene name: fhs
Sequence domains: Formate--tetrahydrofolate ligase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 11 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R32
Unit cell:
a: 160.876Å b: 160.876Å c: 256.118Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.253 0.301
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.
Ragsdale et al. (2008)
4 more

1 mention without citation

Mechanism of N10-formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors.
Celeste et al. (2012)