› 1ei6

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF PHOSPHONOACETATE HYDROLASE COMPLEXED WITH PHOSPHONOFORMATE

Released:
DOI: 10.2210/pdb1ei6/pdb
PDB entry 1ei6 coloured by chain, front view.
PDB entry 1ei6 coloured by chain, side view.
PDB entry 1ei6 coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Entry authors: Holden HM, Benning MM, Dunaway-Mariano D, Kim AD

Function and Biology Details

Reaction catalysed: 
Phosphonoacetate + H(2)O = acetate + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphonoacetate hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 406 amino acids
Theoretical weight: 44.14 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51782 (Residues: 2-407; Coverage: 100%)
Gene name: phnA
Sequence domains: Type I phosphodiesterase / nucleotide pyrophosphatase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand PPF 3 x PPF
Ligand TLA 1 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 57.5Å b: 129.54Å c: 133.38Å
α: 90° β: 96.9° γ: 90°
R-values:
R R work R free
0.194 0.192 0.249
Expression system: Escherichia coli

Quick links