› 1ej2

X-ray diffraction
1.9Å resolution

Crystal structure of methanobacterium thermoautotrophicum nicotinamide mononucleotide adenylyltransferase with bound NAD+

Released:
DOI: 10.2210/pdb1ej2/pdb
PDB entry 1ej2 coloured by chain, front view.
PDB entry 1ej2 coloured by chain, side view.
PDB entry 1ej2 coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
Insights into ligand binding and catalysis of a central step in NAD+ synthesis: structures of Methanobacterium thermoautotrophicum NMN adenylyltransferase complexes.
Saridakis V, Christendat D, Kimber MS, Dharamsi A, Edwards AM, Pai EF
J Biol Chem 276 7225-32 (2001)
PMID: 11063748

Function and Biology Details

Reaction catalysed: 
ATP + nicotinamide ribonucleotide = diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-127825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide-nucleotide adenylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 20.6 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O26253 (Residues: 1-178; Coverage: 100%)
Gene name: MTH_150
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand NA 1 x NA
Ligand NAD 1 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6322
Unit cell:
a: 89.084Å b: 89.084Å c: 109.926Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.241
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Nicotinamide/nicotinic acid mononucleotide adenylyltransferase, new insights into an ancient enzyme.
Zhai et al. (2009)
2 more

7 mentions without citation

Real-time ligand binding pocket database search using local surface descriptors.
Chikhi et al. (2010)
6 more