› 1ejo

X-ray diffraction
2.3Å resolution

FAB FRAGMENT OF NEUTRALISING MONOCLONAL ANTIBODY 4C4 COMPLEXED WITH G-H LOOP FROM FMDV.

Released:
DOI: 10.2210/pdb1ejo/pdb
PDB entry 1ejo coloured by chain, front view.
PDB entry 1ejo coloured by chain, side view.
PDB entry 1ejo coloured by chain, top view.
Source organisms:
Primary publication:
A multiply substituted G-H loop from foot-and-mouth disease virus in complex with a neutralizing antibody: a role for water molecules.
Ochoa WF, Kalko SG, Mateu MG, Gomes P, Andreu D, Domingo E, Fita I, Verdaguer N
J Gen Virol 81 1495-505 (2000)
PMID: 10811933

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
NTP + H(2)O = NDP + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134476 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig kappa chain V-III region PC 3741/TEPC 111 Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.76 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01660 (Residues: 1-111; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q6PF95 (Residues: 22-115, 116-123, 124-234; Coverage: 48%)
Gene name: Igh
Sequence domains:
Structure domains: Immunoglobulins
Capsid protein VP1 Chain: P
Molecule details ›
Chain: P
Length: 15 amino acids
Theoretical weight: 1.63 KDa
Source organism: Foot-and-mouth disease virus C1
Expression system: Not provided
UniProt:
  • Canonical: P15072 (Residues: 859-873; Coverage: 1%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 48.18Å b: 69.328Å c: 146.539Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.262
Expression system: Not provided

Quick links

Citations

2 review citations

Structure and receptor binding.
Jackson et al. (2003)
1 more

5 mentions without citation

X-ray structures of LeuT in substrate-free outward-open and apo inward-open states.
Krishnamurthy et al. (2012)
4 more