› 1el7

X-ray diffraction
1.9Å resolution

COMPLEX OF MONOMERIC SARCOSINE OXIDASE WITH THE INHIBITOR [METHYTELLURO]ACETATE

Released:
DOI: 10.2210/pdb1el7/pdb
PDB entry 1el7 coloured by chain, front view.
PDB entry 1el7 coloured by chain, side view.
PDB entry 1el7 coloured by chain, top view.
Source organism: Bacillus sp. B-0618
Primary publication:
Monomeric sarcosine oxidase: 1. Flavin reactivity and active site binding determinants.
Wagner MA, Trickey P, Chen ZW, Mathews FS, Jorns MS
Biochemistry 39 8813-24 (2000)
PMID: 10913292

Function and Biology Details

Reaction catalysed: 
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.1 KDa
Source organism: Bacillus sp. B-0618
Expression system: Escherichia coli
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
4 bound ligands:
Ligand PO4 3 x PO4
Ligand CL 2 x CL
Ligand MTD 2 x MTD
Ligand TE 2 x TE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 72.722Å b: 69.504Å c: 73.465Å
α: 90° β: 94.12° γ: 90°
R-values:
R R work R free
0.175 0.175 0.213
Expression system: Escherichia coli

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Citations

1 review citation

Recent advances in sustainable nature-based functional materials for biomedical sensor technologies.
Nath et al. (2023)
42 other articles