› 1eqb

X-ray diffraction
2.7Å resolution

X-RAY CRYSTAL STRUCTURE AT 2.7 ANGSTROMS RESOLUTION OF TERNARY COMPLEX BETWEEN THE Y65F MUTANT OF E-COLI SERINE HYDROXYMETHYLTRANSFERASE, GLYCINE AND 5-FORMYL TETRAHYDROFOLATE

Released:
DOI: 10.2210/pdb1eqb/pdb
PDB entry 1eqb coloured by chain, front view.
PDB entry 1eqb coloured by chain, side view.
PDB entry 1eqb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Role of tyrosine 65 in the mechanism of serine hydroxymethyltransferase.
Contestabile R, Angelaccio S, Bossa F, Wright HT, Scarsdale N, Kazanina G, Schirch V
Biochemistry 39 7492-500 (2000)
PMID: 10858298

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 417 amino acids
Theoretical weight: 45.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A825 (Residues: 1-417; Coverage: 100%)
Gene names: JW2535, b2551, glyA
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP

Cofactor: Ligand FFO 4 x FFO
1 bound ligand:
Ligand GLY 4 x GLY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 77.74Å b: 172.48Å c: 95.41Å
α: 90° β: 104.28° γ: 90°
R-values:
R R work R free
0.205 0.205 0.236
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Serine hydroxymethyltransferase revisited.
Schirch et al. (2005)
16 other articles

2 mentions without citation

Impaired folate binding of serine hydroxymethyltransferase 8 from soybean underlies resistance to the soybean cyst nematode.
Korasick et al. (2020)
1 more